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packaging construct pspax2  (Addgene inc)


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    Addgene inc packaging construct pspax2
    Packaging Construct Pspax2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 15719 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pspax2+packaging+construct/psPAX2+(Plasmid+%2312260)/pmc13659691-39-11-14
    Average 98 stars, based on 15719 article reviews
    packaging construct pspax2 - by Bioz Stars, 2026-11
    98/100 stars

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    Related Articles

    Retroviral:

    Article Title: PP2A-activating drugs selectively eradicate TKI-resistant chronic myeloid leukemic stem cells
    Article Snippet: 3-dimensional projections were generated, and the number of positive signals per cell was calculated by ImageJ software (NIH). .. Lentiviral and retroviral transduction Lentiviral pseudotyped particles were produced by transient calcium phosphate transfection (ProFection mammalian transfection System; Promega) of 293T cells with the specific lentiviral vector (18 μg/175 cm 2 ), the psPAX2 packaging construct (Addgene plasmid 12260, 9 μg/175 cm 2 ; provided by D. Trono, Swiss Institute of Technology, Lausanne, Switzerland); and the G-glycoprotein of vesicular stomatitis virus (VSV-G, 1.8 μg/175 cm 2 ). ..

    Transduction:

    Article Title: PP2A-activating drugs selectively eradicate TKI-resistant chronic myeloid leukemic stem cells
    Article Snippet: 3-dimensional projections were generated, and the number of positive signals per cell was calculated by ImageJ software (NIH). .. Lentiviral and retroviral transduction Lentiviral pseudotyped particles were produced by transient calcium phosphate transfection (ProFection mammalian transfection System; Promega) of 293T cells with the specific lentiviral vector (18 μg/175 cm 2 ), the psPAX2 packaging construct (Addgene plasmid 12260, 9 μg/175 cm 2 ; provided by D. Trono, Swiss Institute of Technology, Lausanne, Switzerland); and the G-glycoprotein of vesicular stomatitis virus (VSV-G, 1.8 μg/175 cm 2 ). ..

    Produced:

    Article Title: PP2A-activating drugs selectively eradicate TKI-resistant chronic myeloid leukemic stem cells
    Article Snippet: 3-dimensional projections were generated, and the number of positive signals per cell was calculated by ImageJ software (NIH). .. Lentiviral and retroviral transduction Lentiviral pseudotyped particles were produced by transient calcium phosphate transfection (ProFection mammalian transfection System; Promega) of 293T cells with the specific lentiviral vector (18 μg/175 cm 2 ), the psPAX2 packaging construct (Addgene plasmid 12260, 9 μg/175 cm 2 ; provided by D. Trono, Swiss Institute of Technology, Lausanne, Switzerland); and the G-glycoprotein of vesicular stomatitis virus (VSV-G, 1.8 μg/175 cm 2 ). ..

    Article Title: PP2A-activating drugs selectively eradicate TKI-resistant chronic myeloid leukemic stem cells
    Article Snippet: .. Lentiviral pseudotyped particles were produced by transient calcium phosphate transfection (ProFection mammalian transfection System; Promega) of 293T cells with the specific lentiviral vector (18 μg/175 cm 2 ), the psPAX2 packaging construct (Addgene plasmid 12260, 9 μg/175 cm 2 ; provided by D. Trono, Swiss Institute of Technology, Lausanne, Switzerland); and the G-glycoprotein of vesicular stomatitis virus (VSV-G, 1.8 μg/175 cm 2 ). ..

    Article Title: PP2A-activating drugs selectively eradicate TKI-resistant chronic myeloid leukemic stem cells
    Article Snippet: .. Lentiviral pseudotyped particles were produced by transient calcium phosphate transfection (ProFection mammalian transfection System; Promega) of 293T cells with the specific lentiviral vector (18 μg/175 cm2), the psPAX2 packaging construct (Addgene plasmid 12260, 9 μg/175 cm2; provided by D. Trono, Swiss Institute of Technology, Lausanne, Switzerland); and the G-glycoprotein of vesicular stomatitis virus (VSV-G, 1.8 μg/175 cm2). ..

    Article Title: Inhibition of leukemic stem cells by PP2A activating agents
    Article Snippet: .. Lentiviral pseudotyped particles were produced by transient calcium phosphate transfection (ProFection mammalian transfection System, Promega) of 293T cells with the specific lentiviral vector (18 μg/175 cm2), the psPAX2 packaging construct (Addgene plasmid 12260, provided by Dr. D. Trono, Swiss Institute of Technology, Lausanne, Switzerland; 9 μg/175 cm2) and the G-glycoprotein of vesicular stomatitis virus (VSV-G, 1.8 μg/175 cm2). ..

    Transfection:

    Article Title: PP2A-activating drugs selectively eradicate TKI-resistant chronic myeloid leukemic stem cells
    Article Snippet: 3-dimensional projections were generated, and the number of positive signals per cell was calculated by ImageJ software (NIH). .. Lentiviral and retroviral transduction Lentiviral pseudotyped particles were produced by transient calcium phosphate transfection (ProFection mammalian transfection System; Promega) of 293T cells with the specific lentiviral vector (18 μg/175 cm 2 ), the psPAX2 packaging construct (Addgene plasmid 12260, 9 μg/175 cm 2 ; provided by D. Trono, Swiss Institute of Technology, Lausanne, Switzerland); and the G-glycoprotein of vesicular stomatitis virus (VSV-G, 1.8 μg/175 cm 2 ). ..

    Article Title: PP2A-activating drugs selectively eradicate TKI-resistant chronic myeloid leukemic stem cells
    Article Snippet: .. Lentiviral pseudotyped particles were produced by transient calcium phosphate transfection (ProFection mammalian transfection System; Promega) of 293T cells with the specific lentiviral vector (18 μg/175 cm 2 ), the psPAX2 packaging construct (Addgene plasmid 12260, 9 μg/175 cm 2 ; provided by D. Trono, Swiss Institute of Technology, Lausanne, Switzerland); and the G-glycoprotein of vesicular stomatitis virus (VSV-G, 1.8 μg/175 cm 2 ). ..

    Article Title: PP2A-activating drugs selectively eradicate TKI-resistant chronic myeloid leukemic stem cells
    Article Snippet: .. Lentiviral pseudotyped particles were produced by transient calcium phosphate transfection (ProFection mammalian transfection System; Promega) of 293T cells with the specific lentiviral vector (18 μg/175 cm2), the psPAX2 packaging construct (Addgene plasmid 12260, 9 μg/175 cm2; provided by D. Trono, Swiss Institute of Technology, Lausanne, Switzerland); and the G-glycoprotein of vesicular stomatitis virus (VSV-G, 1.8 μg/175 cm2). ..

    Article Title: Inhibition of leukemic stem cells by PP2A activating agents
    Article Snippet: .. Lentiviral pseudotyped particles were produced by transient calcium phosphate transfection (ProFection mammalian transfection System, Promega) of 293T cells with the specific lentiviral vector (18 μg/175 cm2), the psPAX2 packaging construct (Addgene plasmid 12260, provided by Dr. D. Trono, Swiss Institute of Technology, Lausanne, Switzerland; 9 μg/175 cm2) and the G-glycoprotein of vesicular stomatitis virus (VSV-G, 1.8 μg/175 cm2). ..

    Construct:

    Article Title: PP2A-activating drugs selectively eradicate TKI-resistant chronic myeloid leukemic stem cells
    Article Snippet: 3-dimensional projections were generated, and the number of positive signals per cell was calculated by ImageJ software (NIH). .. Lentiviral and retroviral transduction Lentiviral pseudotyped particles were produced by transient calcium phosphate transfection (ProFection mammalian transfection System; Promega) of 293T cells with the specific lentiviral vector (18 μg/175 cm 2 ), the psPAX2 packaging construct (Addgene plasmid 12260, 9 μg/175 cm 2 ; provided by D. Trono, Swiss Institute of Technology, Lausanne, Switzerland); and the G-glycoprotein of vesicular stomatitis virus (VSV-G, 1.8 μg/175 cm 2 ). ..

    Article Title: PP2A-activating drugs selectively eradicate TKI-resistant chronic myeloid leukemic stem cells
    Article Snippet: .. Lentiviral pseudotyped particles were produced by transient calcium phosphate transfection (ProFection mammalian transfection System; Promega) of 293T cells with the specific lentiviral vector (18 μg/175 cm 2 ), the psPAX2 packaging construct (Addgene plasmid 12260, 9 μg/175 cm 2 ; provided by D. Trono, Swiss Institute of Technology, Lausanne, Switzerland); and the G-glycoprotein of vesicular stomatitis virus (VSV-G, 1.8 μg/175 cm 2 ). ..

    Article Title: The SARS-CoV-2 monoclonal antibody combination, AZD7442, is protective in non-human primates and has an extended half-life in humans
    Article Snippet: .. Pseudovirus was purified from HEK293T cells co-transfected with the psPAX2 packaging construct (AIDS Resource and Reagent Program), luciferase reporter plasmid pLenti-CMV PuroLuc (Addgene), and spike protein expressing plasmid pcDNA3.1-SARS-CoV-2 SΔCT. .. To determine serum neutralizing activity, heat-inactivated serum samples were incubated with pseudovirus before adding to HEK293T-human ACE2 cells for 48 hours, after which cells were lysed using the Steady-Glo Luciferase Assay (Promega).

    Article Title: PP2A-activating drugs selectively eradicate TKI-resistant chronic myeloid leukemic stem cells
    Article Snippet: .. Lentiviral pseudotyped particles were produced by transient calcium phosphate transfection (ProFection mammalian transfection System; Promega) of 293T cells with the specific lentiviral vector (18 μg/175 cm2), the psPAX2 packaging construct (Addgene plasmid 12260, 9 μg/175 cm2; provided by D. Trono, Swiss Institute of Technology, Lausanne, Switzerland); and the G-glycoprotein of vesicular stomatitis virus (VSV-G, 1.8 μg/175 cm2). ..

    Article Title: Inhibition of leukemic stem cells by PP2A activating agents
    Article Snippet: .. Lentiviral pseudotyped particles were produced by transient calcium phosphate transfection (ProFection mammalian transfection System, Promega) of 293T cells with the specific lentiviral vector (18 μg/175 cm2), the psPAX2 packaging construct (Addgene plasmid 12260, provided by Dr. D. Trono, Swiss Institute of Technology, Lausanne, Switzerland; 9 μg/175 cm2) and the G-glycoprotein of vesicular stomatitis virus (VSV-G, 1.8 μg/175 cm2). ..

    Plasmid Preparation:

    Article Title: PP2A-activating drugs selectively eradicate TKI-resistant chronic myeloid leukemic stem cells
    Article Snippet: 3-dimensional projections were generated, and the number of positive signals per cell was calculated by ImageJ software (NIH). .. Lentiviral and retroviral transduction Lentiviral pseudotyped particles were produced by transient calcium phosphate transfection (ProFection mammalian transfection System; Promega) of 293T cells with the specific lentiviral vector (18 μg/175 cm 2 ), the psPAX2 packaging construct (Addgene plasmid 12260, 9 μg/175 cm 2 ; provided by D. Trono, Swiss Institute of Technology, Lausanne, Switzerland); and the G-glycoprotein of vesicular stomatitis virus (VSV-G, 1.8 μg/175 cm 2 ). ..

    Article Title: PP2A-activating drugs selectively eradicate TKI-resistant chronic myeloid leukemic stem cells
    Article Snippet: .. Lentiviral pseudotyped particles were produced by transient calcium phosphate transfection (ProFection mammalian transfection System; Promega) of 293T cells with the specific lentiviral vector (18 μg/175 cm 2 ), the psPAX2 packaging construct (Addgene plasmid 12260, 9 μg/175 cm 2 ; provided by D. Trono, Swiss Institute of Technology, Lausanne, Switzerland); and the G-glycoprotein of vesicular stomatitis virus (VSV-G, 1.8 μg/175 cm 2 ). ..

    Article Title: The SARS-CoV-2 monoclonal antibody combination, AZD7442, is protective in non-human primates and has an extended half-life in humans
    Article Snippet: .. Pseudovirus was purified from HEK293T cells co-transfected with the psPAX2 packaging construct (AIDS Resource and Reagent Program), luciferase reporter plasmid pLenti-CMV PuroLuc (Addgene), and spike protein expressing plasmid pcDNA3.1-SARS-CoV-2 SΔCT. .. To determine serum neutralizing activity, heat-inactivated serum samples were incubated with pseudovirus before adding to HEK293T-human ACE2 cells for 48 hours, after which cells were lysed using the Steady-Glo Luciferase Assay (Promega).

    Article Title: PP2A-activating drugs selectively eradicate TKI-resistant chronic myeloid leukemic stem cells
    Article Snippet: .. Lentiviral pseudotyped particles were produced by transient calcium phosphate transfection (ProFection mammalian transfection System; Promega) of 293T cells with the specific lentiviral vector (18 μg/175 cm2), the psPAX2 packaging construct (Addgene plasmid 12260, 9 μg/175 cm2; provided by D. Trono, Swiss Institute of Technology, Lausanne, Switzerland); and the G-glycoprotein of vesicular stomatitis virus (VSV-G, 1.8 μg/175 cm2). ..

    Article Title: Inhibition of leukemic stem cells by PP2A activating agents
    Article Snippet: .. Lentiviral pseudotyped particles were produced by transient calcium phosphate transfection (ProFection mammalian transfection System, Promega) of 293T cells with the specific lentiviral vector (18 μg/175 cm2), the psPAX2 packaging construct (Addgene plasmid 12260, provided by Dr. D. Trono, Swiss Institute of Technology, Lausanne, Switzerland; 9 μg/175 cm2) and the G-glycoprotein of vesicular stomatitis virus (VSV-G, 1.8 μg/175 cm2). ..

    Virus:

    Article Title: PP2A-activating drugs selectively eradicate TKI-resistant chronic myeloid leukemic stem cells
    Article Snippet: 3-dimensional projections were generated, and the number of positive signals per cell was calculated by ImageJ software (NIH). .. Lentiviral and retroviral transduction Lentiviral pseudotyped particles were produced by transient calcium phosphate transfection (ProFection mammalian transfection System; Promega) of 293T cells with the specific lentiviral vector (18 μg/175 cm 2 ), the psPAX2 packaging construct (Addgene plasmid 12260, 9 μg/175 cm 2 ; provided by D. Trono, Swiss Institute of Technology, Lausanne, Switzerland); and the G-glycoprotein of vesicular stomatitis virus (VSV-G, 1.8 μg/175 cm 2 ). ..

    Article Title: PP2A-activating drugs selectively eradicate TKI-resistant chronic myeloid leukemic stem cells
    Article Snippet: .. Lentiviral pseudotyped particles were produced by transient calcium phosphate transfection (ProFection mammalian transfection System; Promega) of 293T cells with the specific lentiviral vector (18 μg/175 cm 2 ), the psPAX2 packaging construct (Addgene plasmid 12260, 9 μg/175 cm 2 ; provided by D. Trono, Swiss Institute of Technology, Lausanne, Switzerland); and the G-glycoprotein of vesicular stomatitis virus (VSV-G, 1.8 μg/175 cm 2 ). ..

    Article Title: PP2A-activating drugs selectively eradicate TKI-resistant chronic myeloid leukemic stem cells
    Article Snippet: .. Lentiviral pseudotyped particles were produced by transient calcium phosphate transfection (ProFection mammalian transfection System; Promega) of 293T cells with the specific lentiviral vector (18 μg/175 cm2), the psPAX2 packaging construct (Addgene plasmid 12260, 9 μg/175 cm2; provided by D. Trono, Swiss Institute of Technology, Lausanne, Switzerland); and the G-glycoprotein of vesicular stomatitis virus (VSV-G, 1.8 μg/175 cm2). ..

    Article Title: Inhibition of leukemic stem cells by PP2A activating agents
    Article Snippet: .. Lentiviral pseudotyped particles were produced by transient calcium phosphate transfection (ProFection mammalian transfection System, Promega) of 293T cells with the specific lentiviral vector (18 μg/175 cm2), the psPAX2 packaging construct (Addgene plasmid 12260, provided by Dr. D. Trono, Swiss Institute of Technology, Lausanne, Switzerland; 9 μg/175 cm2) and the G-glycoprotein of vesicular stomatitis virus (VSV-G, 1.8 μg/175 cm2). ..

    Purification:

    Article Title: The SARS-CoV-2 monoclonal antibody combination, AZD7442, is protective in non-human primates and has an extended half-life in humans
    Article Snippet: .. Pseudovirus was purified from HEK293T cells co-transfected with the psPAX2 packaging construct (AIDS Resource and Reagent Program), luciferase reporter plasmid pLenti-CMV PuroLuc (Addgene), and spike protein expressing plasmid pcDNA3.1-SARS-CoV-2 SΔCT. .. To determine serum neutralizing activity, heat-inactivated serum samples were incubated with pseudovirus before adding to HEK293T-human ACE2 cells for 48 hours, after which cells were lysed using the Steady-Glo Luciferase Assay (Promega).

    Luciferase:

    Article Title: The SARS-CoV-2 monoclonal antibody combination, AZD7442, is protective in non-human primates and has an extended half-life in humans
    Article Snippet: .. Pseudovirus was purified from HEK293T cells co-transfected with the psPAX2 packaging construct (AIDS Resource and Reagent Program), luciferase reporter plasmid pLenti-CMV PuroLuc (Addgene), and spike protein expressing plasmid pcDNA3.1-SARS-CoV-2 SΔCT. .. To determine serum neutralizing activity, heat-inactivated serum samples were incubated with pseudovirus before adding to HEK293T-human ACE2 cells for 48 hours, after which cells were lysed using the Steady-Glo Luciferase Assay (Promega).

    Expressing:

    Article Title: The SARS-CoV-2 monoclonal antibody combination, AZD7442, is protective in non-human primates and has an extended half-life in humans
    Article Snippet: .. Pseudovirus was purified from HEK293T cells co-transfected with the psPAX2 packaging construct (AIDS Resource and Reagent Program), luciferase reporter plasmid pLenti-CMV PuroLuc (Addgene), and spike protein expressing plasmid pcDNA3.1-SARS-CoV-2 SΔCT. .. To determine serum neutralizing activity, heat-inactivated serum samples were incubated with pseudovirus before adding to HEK293T-human ACE2 cells for 48 hours, after which cells were lysed using the Steady-Glo Luciferase Assay (Promega).

    other:

    Article Title: Trastuzumab Deruxtecan (T-DXd) Resistance via Loss of HER2 Expression and Binding
    Article Snippet: VENTANA DISCOVERY ULTRA instrument was used to perform automatic IHC staining, following the standard protocol.

    Article Title: Identification and Characterization of Two Novel Isoforms of Pirh2 Ubiquitin Ligase That Negatively Regulate p53 Independent of RING Finger Domains
    Article Snippet: Cells were analyzed with an Olympus AX 70 fluorescent microscope, and photographs were captured using a digital camera.

    Article Title: Trastuzumab Deruxtecan (T-DXd) Resistance via Loss of HER2 Expression and Binding
    Article Snippet: Trastuzumab Deruxtecan (T-DXd) Resistance via Loss of HER2 Expression and Binding Wanyi Chen1,2, Avantika Gupta1, Nicholas Mai3, Sharanya Nag1, Joshua S. Lau4, Sukrit Singh5, John D. Chodera2,5, Bo Liu1, Elisa de Stanchina6, Fresia Pareja7, Atif Ali Hashmi7, Miriam Lieberman3, Shanu Modi3, Jacqueline Bromberg3, Pedram Razavi3, Joshua Z Drago3,*, Sarat Chandarlapaty1,2,3,4,* 1Human Oncology and Pathogenesis Program, Memorial Sloan Kettering Cancer Center, New York, USA 2Weill Cornell Graduate School, Weill Cornell Medicine, New York, USA 3Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, USA 4Gerstner Sloan Kettering Graduate School, Memorial Sloan Kettering Cancer Center, New York, USA 5Computational and Systems Biology Program, Memorial Sloan Kettering Cancer Center, New York, USA 6Antitumor Assessment Core Facility, Memorial Sloan Kettering Cancer Center, New York, USA 7Department of Pathology and Laboratory Medicine * Corresponding authors: Joshua Drago & Sarat Chandarlapaty Mailing address: 1275 York Avenue, Box 20, New York, NY 10065, USA Email : dragoj@mskcc.org, chandars@mskcc.org Phone : +1-646-888-4311 Running title: T-DXd resistance on 15 N ovem ber 2025 2 Declaration of interests 1 SC has received personal consulting fees from AstraZeneca, Nuvalent, Daiichi-Sankyo, and 2 Casdin Capital as well as stock options from Totus Medicines. SC has research funding from 3 AstraZeneca and Daiichi Sankyo.. 4 JZD received institutional grants/funding from Merck, AstraZeneca, Bolt Therapeutics, Jazz 5 Pharmaceutics and Orum therapeutics, and has performed consulting/advisory work for 6 AstraZeneca, Genagon, AmMax Bio, NuProbe, Revelio Therpaeutics, MEDACorp, and BAIRD 7 conferences.



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